Brief Description: (Empty Backbone) Plasmid sent as bacteria in agar stab.

Vector backbone: pAAV with pTRIPZ elements

Modifications to backbone: A 1.3-kb filler DNA fragment flanked by two BspQ1 restriction enzyme sites was inserted between the 5′mir and the 3′mir sequences. Once digested with BspQ1, the double-digested vector then has two incompatible 5′ overhang ends and can be easily isolated from any single digested vector, thus eliminating self-ligation background in later procedures. The digested vector is ready for the insertion of the shRNA sequence, which is generated by annealing two single-stranded oligonucleotides to form a small double-stranded insert with proper overhangs as described in the paper.

  • Faculty Lab

           Howard Gu

  • Relevant Publication

Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. Epub 2011 Jun 13.

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